ABSTRACT
Background and Objective: Calcitonin is a small peptide hormone including 32 amino acids and 3.4 KD molecular weight which is produced by the parafollicular cells of the thyroid gland in respond to increasing calcium ions in serum. This peptide is used for adjuvant therapy of osteoporosis, Paget's disease and hypercalcemic shock. In this study, the heterologuse expression of calcitonin was done in Escherichia coli
Methods: In this experimental study, the thioredoxin fusion partner was added to n-terminal of the Salmon calciton in order to increase its stability by the synthetic biology. The recombinant construct was transformed and over expressed into Escherichia coli BL21 [DE3] host cell
Results: SDS-PAGE analysis showed the over expression of recombinant protein after IPTG induction
Conclusion: In this study, the construct including fused Salmon calcitonin gene with thioredoxin was cloned. The SDS-PAGE result showed the stable expression of fused calcitonin
ABSTRACT
Background and Objective: Granulocyte colony-stimulating factors [G-CSF] and its receptor express in developing follicles, fetal and reproductive tissues. The serum G-CSF concentration significantly increases during the ovulatory phase in comparison with other phases, so G-CSF may have an important role in ovulation and the early cross-talk between mother and conceptus in both human and animal models. This study was done to evaluate the Effect of exogenous G-CSF on ovulation and pregnancy rate in NMRI mice
Methods: In this experimental study, 40 mature female and 10 male NMRI mice were randomly allocated into the control and treatment groups. All Ovaries were stimulated with intraperitoneal injections [IP] of 10 IU PMSG and after 48 hour by 10 IU hCG per mouse. The treatment group were recieved G-CSF [50 micro g/kg i.p.], at the time of PMSG administration, while the control group had the same volume of normal saline instead of G-CSF at the same time. 16-18 hours post-hCG administration, twenty female mice of both groups were sacrificed by cervical dislocation and ovulated oocytes were assessed. On day 16 post coitus, the rest of female mice of both groups were scarificed for withdrawing their fetuses to determine the effect of G-CSF on pregnancy rates
Results: The ovulation rate in the treatment group [18.5 +/- 1.25] were significantly more than that of control [12.1 +/- 1.32] [P<0.05]. The number of fetuses had no significant difference between control and treatment groups
Conclusion: This study demonstrated that exogenous G-CSF may affect on folliculogenesis and ovulation but the following pregnancy outcome was not impressed
Subject(s)
Animals, Laboratory , Ovulation/drug effects , Pregnancy Rate , MiceABSTRACT
Background and Objective: Parathyroid proteins involved in calcium homeostasis. With increasing age and other relevant factors, this hormone is not able to perform its role. Using recombinant parathyroid hormone prevent disease progression and effective in improvement of disease. This study was done to design and build the desired construct genes, cloning process and synthesis of soluble parathyroid hormone in E. coli
Methods: In this laboratory study, design and optimization sequence of the gene parathyroid hormone [PTH] was carried out for expression of soluble proteins in bacteria. The construct contining PTH gene [puc 57] transformed into bacteria and cultivation was done in SOB medium then Plasmid extraction was performed. Fragment encoding the PTH was isolated by digestion of the cloning vector and ligate to expression vector [PET-32a]. Subcloning process followed by induction with IPTG 1mM. The recombinant parathyroid hormon was expressed in bacteria, subsequently
Results: After enzymatic digestion, the fragment encoding the protein of interest was properly localized. The process was confirmed by polymerase chain reaction [PCR]. Following performing a transformation, induction process performed by IPTG with final concentration 1mM that caused the soluble parathyroid proteins to be expressed in bacteria and the process was confirmed by Western blot technique
Conclusion: Protein expression in bacteria due to its rapid growth and the need to inexpensive medium is cost-effective. Soluble recombinant protein expression reduces downstream of recombinant protein production
ABSTRACT
probably after pestilence, HIV/AIDS is most important and severe health threat for public health. Prisons could facilitate the spread of HIV/AIDS and Hepatitis and transmit it to societies. The main strategy to control HIV/AIDS and hepatitis among Injecting Drug Users is Harm Reduction Programs. These programs are conducted in Iran Prisons. The goal of this study is to evaluate Harm reduction Programs including Methadone Maintenance Therapy [MMT] and Needle exchange from the viewpoint of prison staff in Iran including principals, managers, physicians and counselors. It was a cross sectional study in which 370 prison staff working in prisons all around the country were evaluated to assess their viewpoint and insight about Harm reduction Programs including Methadone Maintenance therapy and Needle exchange. A percentage of 95.23 of prison managers and experts had a positive view toward Methadone Maintenance therapy. 70.25 percent had a positive insight toward needle exchange program. The employment type and work experience was significantly [p<0.05] related to the viewpoint of managers and experts towards Methadone Maintenance therapy program. Age was the only factor that had a significant relationship [p<0.05] with Needle exchange program. MMT has lots of supporters in prisons. The positive insights of managers and experts towards methadone maintenance therapy program demonstrate the high level of staff's approval for conducting this program; it could be interpreted as a sign of success of program in the country. However there is not a comprehensive approval and collaboration with needle exchange program in prisons. Nevertheless, it is alarming that 42.5% of staff believe that conducting methadone maintenance therapy may increase and intensify addiction in prisons
ABSTRACT
Background: Foot drop is a state that patient unable to be dorsiflexion or extension of foot fingers. External pressure is the most important cause of this neuropathy. Among soldiers, following the heavy training, this complication may occur that cause frequent reference of soldiers to military hospitals wasting human force and economic load. Therefore, we decided to evaluate frequency of this disease and find methods to prevent
Materials and Methods: This study is a descriptive case series that performed by studing hospital files of 42 patients during the years 2000 to 2003 and the data was analyzed by SPSS-11.5 software
Results: Twenty-eight [66.66%] patients had training for one month, 12 patients had [28.57%] between 1-3 month and 2 patients had [4.77%] longer of 3 month. Six cases [14.29%] had complete injury and 36 cases had incomplete injury. Seven cases [16.67%] had complete recovery and 35 cases [83.33%] had incomplete injury and none had paralysis in any case didn't have. Changes in NCV was detected but EMG was Normal
Conclusions: Trauma following to military training probably due to squatting leads to foot drop